Staining Reagents
Lifeline Cell Technology CLSTN ALZRD OSTEO LIFELINE BTL
Lifeline's 2% Alizarin Red Stain is used to stain calcium deposited by osteocytes.
Electron Microscopy Sciences Basic Fuchsin 0.25% Aqueous 500 ML
Used in Brown and Brenn Method for gram positive and gram negative Bacteria.
Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References are Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931) and AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222
Electron Microscopy Sciences Acid Fuchsin 0.5% Aqueous 500 ML
Acid Fuchsin Aqueous solution is a prepared, ready-to-use, high quality staining solution that saves time and effort and produces consistent uniform results.
Electron Microscopy Sciences Methylene Blue Solution 1% 500 ML
Used in Pinkerton's Method for Rickettsia, For inclusion body stain. Aee Simmons, J.S. and Gentzkow, C.J., Laboratory Methods of the United States Army 5th Ed., Lee & Febiger, Philadelphia, p. 572, 1944 or Luna, L.G., (ed) Manual of Histologic Staining Methods or the AFIP, 3rd Ed., McGraw-Hill, N.Y., . 237, c 1968
Volu Sol Wright Stain Kit, 8 oz / 250 mL, Volu-Sol™
Wright and Wright-Giemsa Stain Kits include three components: a stain, buffer, and a rinse. Stain peripheral blood and bone marrow smears. Used to perform differential white blood cell counts and to study red blood cell morphology. Abnormal granulocyte, lymphocyte or monocyte cell counts may be used to facilitate the diagnosis of diseases such as leukemia or bacterial infections
Electron Microscopy Sciences Mayer Hematoxylin 500 ML
References: AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed., New York, McGraw-Hill Publications, c. 1968, p. 140.
Volu Sol Papanicolaou Stain, OG-6, 128 oz / 3.78 L, Volu-Sol™
Papanicolaou stain used for critical examination of nuclei and cytoplasmic components of exfoliative cells
Volu Sol Gill's Hematoxylin I (Single Strength), 128 oz / 3.78 L, Volu-Sol™
Nuclear stain, progressive. Used with hematoxylin and eosin staining
Sigma Aldrich Fine Chemicals Biosciences Trichrome Stain (Masson) Kit
May be useful in study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes. Collagen and muscle fiber stain differentially when treated sequentially with Biebrich Scarlet-Acid Fuchsin, PTA/PMA and Aniline Blue. Cytoplasm and muscle fibers stain red whereas collagen displays blue coloration.
Electron Microscopy Sciences Giemsa Stock Solution 250 ML
Used in Differentiation of leucocytes, rickettsia, bacteria and inclusion bodies. Nuclei, additional leukocyte features are similar. References: Clark, G., Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., p. 125, c. 1973
Electron Microscopy Sciences Giemsa Stock Solution 250 ML
Used in Wolbach's Giemsa Method Wolbach, Todd, and Palfrey (1922) Nuclei, collagen. References are Wolbach, S.B., Todd, J.L. and Palfrey, F.W., The Etilogy of Pathology Typhus, Harvard University Press, Cambridge, MA., p. 13 – 14,c 1922 and Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, Y, p. 119, c. 1968
Electron Microscopy Sciences Aldehyde Fuchsin 250 ML
Used in Gridley's Method for Fungi for stain fungal mycelia, conidia, morphological detail of yeast forms and hyphae. See gridley, M.F.: American J. Clin. Path., 23:303 (1953) or AFIP Manual of Histologic Staining Techniques: 3rd., Ed. ED. L. Luna, New York: McGraw-Hill Publications, c. 1968, p. 229
Electron Microscopy Sciences Crystal Violet 1% Aqueous 500 ML
Crystal Violet solution is a prepared, ready-to-use, high quality staining solution that saves time and effort and produces consistent uniform results.
Sigma Aldrich Fine Chemicals Biosciences BioTracker ferroOrange Liv
Iron the most abundant transition metal in our bodies is involved in a number of biologically important processes such as respiration oxygen transport and energy production in collaboration with oxygen. In living cells iron exists mainly as ferrous (fe2) and ferric (fe3) ionic forms. As a major contributor to oxidative damage of cells fe2 is implicated in serious diseases such as cancers and neurodegenerative disorders because of its ability to produce harmful reactive oxygen species via contact with oxygen superoxide and hydrogen peroxide (H2O2).BioTracker ferroOrange Live Cell Dye is an orange fluorescent probe that specifically detects labile iron (II) ions (fe2) only. The intensity of fluorescence does not increase in the presence of iron (III) ions (fe3) or bivalent metal ions other than iron. It also does not react to chelated iron in ferritin and other substances. ferroOrange is suitable for live-cell imaging because it is highly cell-permeable and has low cell toxicity